Short answer: no.

On 64-bit versions of Windows Server 2003, the system automatically expands the virtual address space available to 32-bit user-mode programs linked with the /LARGEADDRESSAWARE option as needed without the /3GB boot parameter. On Windows Server 2003 RTM (without Service Pack 1), these 32-bit programs can access up to 3 GB of virtual address space. On Windows Server 2003 with Service Pack 1, they can access up to 4 GB of virtual address space. Native 64-bit user-mode programs can access up to 8 TB of virtual address space

Source: http://smtp25.blogspot.com/2007/07/do-i-need-3gb-switch-on-64bit-platform.html